KMID : 0861020140290060117
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Korea Journal of Herbology 2014 Volume.29 No. 6 p.117 ~ p.123
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Anti-inflammatory effect of Sinhyowoldo-san Extract with regard to Pro-inflammatory Mediators in PMA plus A23187-induced Human Mast Cells
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Wi Gyeong
Yang Da-Wun Kang Ok-Hwa Kim Sung-Bae Mun Su-Hyun Seo Yun-Soo Kang Da-Hye Lim Jae-Soo Kim Ma-Ryong Kwak Nam-Won Kong Ryong Kwon Dong-Yeul
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Abstract
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Objectives : Sinhyowoldo-san (SHWDS) is said to be a traditional medicine used for shigellosis, abdominal pain, diarrhea. But mechanism of SHWDS mediated-modulation of immune function is not sufficiently understood. To ascertain the molecular mechanisms of SHWDS 70% EtOH extract on pharmacological and biochemical actions in inflammation, we researched the effect of pro-inflammatory mediators in phorbol-12-myristate-13-acetate (PMA)+ A23187-activated human mast cell line (HMC-1).
Methods : In the present research, cell viability was measured by MTS assay. pro-inflammatory cytokine production was measured by performing enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR), and western blot analysis to analyze the activation of mitogen-activated protein kinases (MAPKs), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-†B). The investigation focused on whether SHWDS inhibited the expressions of interleukin-6 (IL-6), interleukin-8 (IL-8), MAPKs and NF-†B in PMA+A23187-activated HMC-1 cells.
Results : SHWDS has no cytotoxicity at measured concentration (50, 100, and 250 ¥ìg/ml). SHWDS (250 ¥ìg/ml) inhibits pro-inflammatory cytokine expression in PMA+ A23187-activated HMC-1 cells. Moreover, SHWDS inhibited cyclooxygenase (COX)-2 expression. In activated HMC-1 cells, SHWDS suppressed phosphorylation of extracellular signal-regulated kinase (ERK 1/2) and c-jun N-terminal Kinase (JNK 1/2). Then, SHWDS suppressed activation of nuclear factor NF-¬ÜB in nuclear, degradation of IkB ¥á in cytoplasm.
Conclusions : We propose that SHWDS has an anti-inflammatory therapeutic potential, which may result from inhibition of ERK 1/2, JNK 1/2 phosphorylation and NF-†B activation, thereby decreasing the expression of pro-inflammatory genes.
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KEYWORD
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Sinhyowoldo-san, Anti-inflammatory activity, Human mast cell, Mitogen-activated protein kinases, MAPKs, Nuclear factor NF-†B, Cytokines
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